Studies on Tissue Uptake of Insulin in Man Using a Differential Immunoassay for Endogenous
نویسنده
چکیده
The radio-immunological technique for assaying endogenous plasma insulin in man recently described by Yalow and Berson (1, 2) promises to be of considerable value in the investigation of insulin metabolism. Other immunological methods that have been reported are not so sensitive as this method, since they fail to detect insulin in fasting normal human plasma (3). In contrast to the bioassay methods, which are limited to the estimation of "insulin-like" activity, the immunoassay is specific for insulin. The present communication describes a radio-immunological procedure for assaying endogenous (human) and exogenous (beef) insulin separately when both are present in human plasma. This differential assay is based on the observation that beef and human insulin react differently with antibeef insulin-binding antibodies in antisera from guinea pigs and man (1, 4). First, the plasma beef insulin level is measured with a relatively speciesspecific human antibeef insulin serum, which reacts poorly with human insulin. It is then possible to assay the plasma endogenous insulin with a guinea pig antibeef insulin serum which reacts strongly with both beef and human insulin. With this method it has been possible to demonstrate that, in man, both the liver and peripheral tissues remove endogenous and exogenous insulin from the blood.
منابع مشابه
Studies on tissue uptake of insulin in man using a differential immunoassay for endogenous and exogenous insulin.
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تاریخ انتشار 2013